Research Article Open Access

Analysis of bacterial degradation of Azo dye Congo Red using HPLC

Abstract

The efficiency of four bacterial species namely Enterobacter cloacae, Hafnia alvei, Klebsiella pneumoniae, Serratia marcescens isolated from agricultural soil in degrading the azo dye Congo Red was analyzed. The bio-degradation was monitored using HPLC (High Performance Liquid Chromatography). Appearance of new peaks and disappearance of certain peaks were observed which showed that either there is bio-degradation or bio-transformation of the azo dye. Sample treated with H. alvei obtained the peak with shortest retention time (RT), 1.98. Highest retention time and thus the lowest polarity were obtained in the sample treated with H. alvei and E. cloacae. There was synergistic effect upon dye degradation since the sample treated by all the bacterial species in combination showed better degradation than that treated by individual bacteria.

D. Selva Raj, R. Jaisy Prabha and R. Leena

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