ISSN (0970-2083)

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Research Article Open Access

Degradation Of Orange G By Immobilized Bacillus Sp. S2

Abstract

 Orange G, asulfonated azo dye, is used in silk, wool products and dyeing. It is recalcitrant to oxidative degradation, and persists in aquatic environment. In the present study, Orange G dye degrading bacterial strain was isolated from soil containing rusted iron shavings. The present study illustrates the ability of a bacterial strain S2, to degrade the Orange G. The strain was identified as Bacillus sp. based on chemical and phylogenetic analysis. The study presents comparative assessment of degradation of Orange G by free and immobilized cells of Bacillus sp. S2. The dye degradation was confirmed through UV-visible spectroscopy, HPLC and GC-MS analysis. Spectral analysis of the product following dye degradation showed that the medium did not contain any aromatic compounds. In the decolorization study, the strain showed 100% degradation of dye at 100 mgL-1 dye concentration by free cells at 24 h incubation, pH 7.0 and temperature 37o C whereas immobilized Ca-alginate beads with strain showed 100 % degradation up to 250 mg L-1 of dye concentration under similar conditions. In packed bed reactor, highest dye degradation was obtained at lower flow rate. The study could be used for the development Ca-alginate immobilized bacterial cells in wastewater treatment plants.

 Uttam Kumar And  V.K. Mishra                

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