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The present study was conducted to quantitatively measure the levels of Cr, Pb, Cd, As, Ni and Hg in soil, fodder and meat samples.The study area chosen for the present study is sewage polluted River Vaigai flowing sites. Two sites were chosen for this work, Site 1 (Vaigai vadakarai) and Site 2 (Aarapalayam). Fresh samples were collected from the study area. Heavy metals like Cr, Cd, Pb, As, Ni and Hg were analysed using atomic absorption spectrophotometer (AAS). In the present study Pb, Cr, Cd, Ni and Hg concentration in Site 1 was high when compare with Site 2, except As which shows high concentration in Site 2. This result indicates Site 1 in heavily polluted than Site 2. Results of meat sample shows that Pb, Cd, Cr and Hg values are higher than the permissible limit, especially Ni. In the same way the results of soil sample shows Cd, Pb, Cr, Hg and Ni levels were higher than the allowed limits. Pb concentration was very higher than other metals. In fodder sample the levels of Cd, As and Hg are higher than the permissible limit.