Molecular Characterization and Phylogenetic Analysis of Metallothionein Gene In Catla Catla

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Abstract

Metallothionein gene (M1) has been used commonly as a potential molecular marker to detect the harmful effects of heavy metals in aquatic environment. Fish(Catta ratio) was exposed to sliver nanoparticles for induction of MT gene. Gene MT was successfully characterized and 179bp amplicon product was amplified. MT-2 gene, isolated from the liver, was highly similar to the Cyprinus arsine metallothionein gene. The particle size of the sliver nanopartkles were found to be in the range of 70nm to %Snot with an average size of 192 nm. All the above results revealed thatfish MT gene would be a useful blomarker for metal pollution.